Journal: Journal of Nanobiotechnology

Article Title: Design of RGD-functionalized GSH-responsive pegylated polymeric protacs for selective BRD4 degradation and EndMT-driven cardiac fibrosis inhibition

doi: 10.1186/s12951-026-04036-7

Figure Lengend Snippet: RGD-PEG-MZ1 exerted a profound inhibitory effect on the EndMT in vitro. ( A-D ) The expression of Collagen-I, Fibronectin, VE-cadherin, α-SMA and FSP1 was assessed using western blotting (WB) in HUVECs and HMEC-1. ( E ) Immunofluorescence staining for α-SMA (green) and VE-cadherin (green) in HUVECs. Scale bar, 100 μm. The data are presented as the Mean ± SD of three independent experiments. # p < 0.05 and ## p < 0.01 vs. NC group; ns, p > 0.05 vs. control group; * p < 0.05 and ** p < 0.01 vs. control group (one-way ANOVA followed by Bonferroni post hoc test)

Article Snippet: The slides were initially stabilized with a 5% goat serum solution, then exposed to primary antibodies—α-SMA (Proteintech, 14395-1-AP), Collagen-I (Proteintech, 14695-1-AP), CD31 (Proteintech, 66065-2-Ig), Raf1 (Abcam, ab181115), Raf1 (phospho S259) (Abcam, ab173539), ERK1 + ERK2 (Abcam, ab184699) and ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) (Abcam, ab278538)—and subsequently incubated at 4 °C for an extended period.

Techniques: In Vitro, Expressing, Western Blot, Immunofluorescence, Staining, Control

Journal: EMBO Molecular Medicine

Article Title: Characterization and therapy of fertilization failure in murine and human models with HNRNPR mutations

doi: 10.1038/s44321-026-00374-z

Figure Lengend Snippet: ( A ) MII oocyte and zygote fluorescence imaging reflecting fertilization after ICSI of Hnrnpr -mutated sperm. Formation of male and female pronuclei indicates successful fertilization. Tubulin labels the spindle, showing that the oocyte is at metaphase of meiosis II, and P1 marks the first polar body. The white dotted line defines the boundary of the zygotes. Scale bar = 10 μm. ( B ) Quantification of the percentage of zygotes from ( A ). Data are presented as mean ± s.d., and P values were calculated using a two-sided Student’s t test. Data in ( A , B ) represent results from six independent experiments (two technical and three biological replicates, n = 6).

Article Snippet: Rabbit anti-alpha Tubulin , MedChemExpress , Cat# HY- P86200.

Techniques: Fluorescence, Imaging

Journal: Journal of Carcinogenesis

Article Title: Clinicopathological significance of immunohistochemical expression of Filamin A in breast cancer

doi: 10.4103/jcar.JCar_9_20

Figure Lengend Snippet: Association of Filamin A expression with clinicopathological parameters

Article Snippet: Four-micron thick sections are cut from paraffin-embedded tissue blocks and were subjected to immunohistochemistry with anti-Filamin A/FLNa Picoband rabbit IgG polyclonal antibody (Boster Biological Technology, Pleasanton, CA, USA) in lyophilized form at dilution of 0.5 μg/ml with antibody diluent.

Techniques: Expressing

Journal: Journal of Carcinogenesis

Article Title: Clinicopathological significance of immunohistochemical expression of Filamin A in breast cancer

doi: 10.4103/jcar.JCar_9_20

Figure Lengend Snippet: Left: Photomicrograph showing Filamin A expressivity in nonneoplastic breast tissue showing membranous and cytoplasmic positivity (×10). Right: Photomicrograph showing a membranous pattern in myoepithelial cells in fibroadenoma (×10)

Article Snippet: Four-micron thick sections are cut from paraffin-embedded tissue blocks and were subjected to immunohistochemistry with anti-Filamin A/FLNa Picoband rabbit IgG polyclonal antibody (Boster Biological Technology, Pleasanton, CA, USA) in lyophilized form at dilution of 0.5 μg/ml with antibody diluent.

Techniques:

Journal: Journal of Carcinogenesis

Article Title: Clinicopathological significance of immunohistochemical expression of Filamin A in breast cancer

doi: 10.4103/jcar.JCar_9_20

Figure Lengend Snippet: Left: Photomicrograph showing invasive carcinoma of the breast (H and E, ×40). Right: Immunohistochemistry: Score 0 (absent) (×40) photomicrograph showing the absence of Filamin A expression in malignant cells

Article Snippet: Four-micron thick sections are cut from paraffin-embedded tissue blocks and were subjected to immunohistochemistry with anti-Filamin A/FLNa Picoband rabbit IgG polyclonal antibody (Boster Biological Technology, Pleasanton, CA, USA) in lyophilized form at dilution of 0.5 μg/ml with antibody diluent.

Techniques: Immunohistochemistry, Expressing

Journal: Journal of Carcinogenesis

Article Title: Clinicopathological significance of immunohistochemical expression of Filamin A in breast cancer

doi: 10.4103/jcar.JCar_9_20

Figure Lengend Snippet: Left: Photomicrograph showing invasive carcinoma of the breast (H and E, ×40). Right: Immunohistochemistry: Score 3 (strong positivity) (×40); photomicrograph showing strong cytoplasmic positivity of Filamin A expression in malignant cells

Article Snippet: Four-micron thick sections are cut from paraffin-embedded tissue blocks and were subjected to immunohistochemistry with anti-Filamin A/FLNa Picoband rabbit IgG polyclonal antibody (Boster Biological Technology, Pleasanton, CA, USA) in lyophilized form at dilution of 0.5 μg/ml with antibody diluent.

Techniques: Immunohistochemistry, Expressing